Lysate prepared from rat liver tissue, rat thymus tissue, rat lung tissue and rat pancreas tissue. Whole cell lysate prepared from HT1080 cells, MM453 cells, SMMC cells, 6T-CEM cells and Colo320 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.
FunctionRecognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
Tissue specificityLiver and promyelocytic leukemia cell line HL60.
All lanes : Anti-Hex antibody (ab95438) at 1 µg/ml
Lane 1 : Lysate prepared from rat liver tissue. Lane 2 : Lysate prepared from rat thymus tissue. Lane 3 : Lysate prepared from rat lung tissue. Lane 4 : Lysate prepared from rat pancreas tissue. Lane 5 : Whole cell lysate prepared from HT1080 cells. Lane 6 : Whole cell lysate prepared from MM453 cells. Lane 7 : Whole cell lysate prepared from SMMC cells. Lane 8 : Whole cell lysate prepared from 6T-CEM cells. Lane 9 : Whole cell lysate prepared from Colo320 cells.