Overview

  • Product name
  • Description
    Rabbit polyclonal to Hex
  • Specificity
    ab95438 shows no cross reactivity with other proteins. The immunogen is different from the mouse sequence by one amino acid.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 24-39 (

    LQPAHPTPFYIEDILG

    ) of Human Hex. Uniprot ID = Q03014.

  • Positive control
    • Lysate prepared from rat liver tissue, rat thymus tissue, rat lung tissue and rat pancreas tissue. Whole cell lysate prepared from HT1080 cells, MM453 cells, SMMC cells, 6T-CEM cells and Colo320 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab95438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • Function
    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
  • Tissue specificity
    Liver and promyelocytic leukemia cell line HL60.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed during hematopoiesis.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hematopoietically expressed homeobox antibody
    • Hematopoietically-expressed homeobox protein HHEX antibody
    • HEX antibody
    • HHEX antibody
    • HHEX_HUMAN antibody
    • HMPH antibody
    • Homeobox hematopoietically expressed antibody
    • Homeobox protein HEX antibody
    • Homeobox protein PRH antibody
    • HOX11L PEN antibody
    • PRH antibody
    • PRHX antibody
    • Proline rich homeodomain containing transcription factor antibody
    see all

Images

  • All lanes : Anti-Hex antibody (ab95438) at 1 µg/ml

    Lane 1 : Lysate prepared from rat liver tissue.
    Lane 2 : Lysate prepared from rat thymus tissue.
    Lane 3 : Lysate prepared from rat lung tissue.
    Lane 4 : Lysate prepared from rat pancreas tissue.
    Lane 5 : Whole cell lysate prepared from HT1080 cells.
    Lane 6 : Whole cell lysate prepared from MM453 cells.
    Lane 7 : Whole cell lysate prepared from SMMC cells.
    Lane 8 : Whole cell lysate prepared from 6T-CEM cells.
    Lane 9 : Whole cell lysate prepared from Colo320 cells.


    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

ab95438 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab95438.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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