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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-Hex antibody - ChIP Grade
See all Hex products (7) ...
Description
Rabbit polyclonal to Hex - ChIP Grade
Tested applications
ChIP, WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex.
(Peptide available as ab28465.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) HEK293 Human embryonic kidney cell line SHSY-5Y (Human neuroblastoma cell line) HepG2 (Human hepatocellular liver carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Mesoderm
Developmental Biology >> Embryogenesis >> Axis formation and symmetry
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Lineage Markers >> Mesoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
ChIP: Use at an assay dependent dilution.
IHC-FoFr: 1/100 (See abreview).
WB: 1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
Tissue specificity
Liver and promyelocytic leukemia cell line HL60.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed during hematopoiesis.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ03014
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Database links
- Entrez Gene: 3087 Human
- Entrez Gene: 15242 Mouse
- Entrez Gene: 397232 Pig
- Entrez Gene: 79237 Rat
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: P43120 Mouse
- SwissProt: Q9R1X2 Mouse
see all
Anti-Hex antibody - ChIP Grade images:
Western blot - Hex antibody (ab34222)
All lanes : Anti-Hex antibody - ChIP Grade (ab34222) at 1/250 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Western blot - Hex antibody (ab34222)
All lanes : Anti-Hex antibody - ChIP Grade (ab34222) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Hex antibody (ab34222)
ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet
References for Anti-Hex antibody - ChIP Grade (ab34222)
ab34222 has not yet been referenced specifically in any publications.
Publishing research using ab34222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"