Anti-Hex antibody - ChIP Grade (ab34222)


  • Product nameAnti-Hex antibody - ChIP Grade
    See all Hex primary antibodies
  • Description
    Rabbit polyclonal to Hex - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex.

    (Peptide available as ab28465.)

  • Positive control
    • This antibody gave a positive signal in Mouse Liver Tissue Lysate.


Associated products


Our Abpromise guarantee covers the use of ab34222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465).
IHC-FoFr 1/100.


  • FunctionRecognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
  • Tissue specificityLiver and promyelocytic leukemia cell line HL60.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed during hematopoiesis.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hematopoietically expressed homeobox antibody
    • Hematopoietically-expressed homeobox protein HHEX antibody
    • HEX antibody
    • HHEX antibody
    • HHEX_HUMAN antibody
    • HMPH antibody
    • Homeobox hematopoietically expressed antibody
    • Homeobox protein HEX antibody
    • Homeobox protein PRH antibody
    • HOX11L PEN antibody
    • PRH antibody
    • PRHX antibody
    • Proline rich homeodomain containing transcription factor antibody
    see all

Anti-Hex antibody - ChIP Grade images

  • Anti-Hex antibody - ChIP Grade (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

References for Anti-Hex antibody - ChIP Grade (ab34222)

ab34222 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (16HBE14o- cells and Caco2 cells)
Specification 16HBE14o- cells and Caco2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 12 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34222 and one vial of ab13537.

To check the status of the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step Real-time PCR
Positive control region known to bind this transcription factor
Negative control region known to be devoid of this transcription factor

Abcam user community

Verified customer

Submitted Jan 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

Submitted Jun 12 2009