Anti-Hex antibody [HHE5I261] (ab50836)

Overview

• Product name
  Anti-Hex antibody [HHE5I261]
  See all Hex primary antibodies
• Description
  Mouse monoclonal [HHE5I261] to Hex
• Specificity
  This antibody reacts with Hex.
• Tested applications
  Suitable for: WB, ELISAmore details
• Species reactivity
  Reacts with: Recombinant fragment
  Predicted to work with: Human
• Immunogen

  Hex recombinant fragment (Human), containing 50-200 AAs from near the N-terminal.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
• Storage buffer
  Preservative: 0.05% Sodium Azide
  Constituents: 1% PBS, BSA
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane.
• Clonality
  Monoclonal
• Clone number
  HHE5I261
• Isotype
  IgG1
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

  • Stem Cells
  • Lineage Markers
  • Mesoderm

  • Developmental Biology
  • Embryogenesis
  • Axis formation and symmetry

  • Developmental Biology
  • Lineage specification
  • Mesoderm

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Function
  Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
• Tissue specificity
  Liver and promyelocytic leukemia cell line HL60.
• Sequence similarities
  Contains 1 homeobox DNA-binding domain.
• Developmental stage
  Expressed during hematopoiesis.
• Cellular localization
  Nucleus.

• Target information above from: UniProt accession Q03014 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 3087 Human
  • Omim: 604420 Human
  • SwissProt: Q03014 Human
  • Unigene: 118651 Human
• Alternative names
  • Hematopoietically expressed homeobox antibody
  • Hematopoietically-expressed homeobox protein HHEX antibody
  • HEX antibody

  • HHEX antibody
  • HHEX_HUMAN antibody
  • HMPH antibody
  • Homeobox hematopoietically expressed antibody
  • Homeobox protein HEX antibody
  • Homeobox protein PRH antibody
  • HOX11L PEN antibody
  • PRH antibody
  • PRHX antibody
  • Proline rich homeodomain containing transcription factor antibody

  see all

Protocols

• Western blot protocols