Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human HEXB.
Our Abpromise guarantee covers the use of ab107254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HEXB knockout HAP1 whole cell lysate (20 µg)
Lane 3: HEK293 whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab107254 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab107254 was shown not to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. Ab107254 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab107254 has not yet been referenced specifically in any publications.