• Product nameAnti-HEXB antibody
    See all HEXB primary antibodies
  • Description
    Rabbit polyclonal to HEXB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Zebrafish, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human HEXB.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; HEK293; HepG2; Caco2; MCF7.



Our Abpromise guarantee covers the use of ab107254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).


  • FunctionResponsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
  • Involvement in diseaseDefects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2) [MIM:268800]; also known as Sandhoff disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2-gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 20 family.
  • Post-translational
    N-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively.
    The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta hexosaminidase beta chain antibody
    • Beta hexosaminidase subunit beta antibody
    • Beta N acetylhexosaminidase antibody
    • Beta-hexosaminidase subunit beta chain A antibody
    • Beta-N-acetylhexosaminidase subunit beta antibody
    • Cervical cancer proto oncogene 7 protein antibody
    • Cervical cancer proto-oncogene 7 protein antibody
    • ENC 1AS antibody
    • Epididymis luminal protein 248 antibody
    • HCC 7 antibody
    • HCC-7 antibody
    • HCC7 antibody
    • HEL 248 antibody
    • HEX B antibody
    • Hexb antibody
    • HEXB_HUMAN antibody
    • Hexosaminidase B (beta polypeptide) antibody
    • Hexosaminidase B antibody
    • Hexosaminidase subunit B antibody
    • HexosaminidaseB antibody
    • N acetyl beta glucosaminidase antibody
    • N-acetyl-beta-glucosaminidase subunit beta antibody
    see all

Anti-HEXB antibody images

  • Predicted band size : 63 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HEXB knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HEK293 whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab107254 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab107254 was shown not to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. Ab107254 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-HEXB antibody (ab107254) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
    HEXB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References for Anti-HEXB antibody (ab107254)

ab107254 has not yet been referenced specifically in any publications.

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We only have 1 HEXB protein in our catalogue as ab114915, but it has not been tested for its activity.


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