The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20000.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method, boiling for 2-3 min, before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionResponsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Involvement in diseaseDefects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2) [MIM:268800]; also known as Sandhoff disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2-gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula.
Sequence similaritiesBelongs to the glycosyl hydrolase 20 family.
Post-translational modificationsN-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively. The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using HEXB antibody (ab65001) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising peptide. Antigen retrieval was performed by boiling in a pressure cooker for 2-3 min. A Polymer system was used for signal enhancing. Stained using DAB.
Western blot - HEXB antibody (ab65001)
All lanes : Anti-HEXB antibody (ab65001) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells Lane 2 : Extracts from Jurkat cells with immunizing peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 63 kDa Observed band size : 63 kDa
References for Anti-HEXB antibody (ab65001)
has not yet been referenced specifically in any publications.
Publishing research using ab65001? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"