The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method, boiling for 2-3 min, before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Involvement in disease
Defects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2) [MIM:268800]; also known as Sandhoff disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2-gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula.
Belongs to the glycosyl hydrolase 20 family.
N-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively. The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using HEXB antibody (ab65001) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising peptide. Antigen retrieval was performed by boiling in a pressure cooker for 2-3 min. A Polymer system was used for signal enhancing. Stained using DAB.
Western blot - HEXB antibody (ab65001)
All lanes : Anti-HEXB antibody (ab65001) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells Lane 2 : Extracts from Jurkat cells with immunizing peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 63 kDa Observed band size : 63 kDa