Overview

  • Product name
  • Description
    Sheep polyclonal to HEXIM1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with 6x His tag

Properties

Applications

Our Abpromise guarantee covers the use of ab28016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IP Use at an assay dependent dilution.

Target

  • Function
    Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
  • Tissue specificity
    Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
  • Sequence similarities
    Belongs to the HEXIM family.
  • Domain
    The coiled-coil domain mediates oligomerization.
  • Cellular localization
    Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac lineage protein 1 antibody
    • CLP 1 antibody
    • CLP1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • Estrogen down-regulated gene 1 protein antibody
    • FLJ13562 antibody
    • Hexamethylene bis acetamide inducible 1 antibody
    • Hexamethylene bis acetamide inducible protein antibody
    • Hexamethylene bis acetamide inducible transcript 1 antibody
    • Hexamethylene bis-acetamide-inducible protein 1 antibody
    • HEXI1_HUMAN antibody
    • HEXIM 1 antibody
    • Hexim1 antibody
    • HEXIM1 protein antibody
    • HIS 1 antibody
    • HIS1 antibody
    • HMBA inducible antibody
    • MAQ 1 antibody
    • MAQ1 antibody
    • Menage a quatre 1 antibody
    • Menage a quatre protein 1 antibody
    • Protein HEXIM1 antibody
    see all

Anti-HEXIM1 antibody images

  • All lanes : Anti-HEXIM1 antibody (ab28016) at 1/1000 dilution

    Lane 1 : recombinant 6x His tagged HEXIM1 protein
    Lane 2 : Hela whole cell lysate
    Lane 3 : Hela cell nuclear lysate

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 41 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-HEXIM1 antibody (ab28016)

This product has been referenced in:
  • Brogie JE & Price DH Reconstitution of a functional 7SK snRNP. Nucleic Acids Res 45:6864-6880 (2017). Read more (PubMed: 28431135) »
  • Safronova OS  et al. Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to promote gene-specific transcriptional repression. Nucleic Acids Res 42:8954-69 (2014). IP ; Human . Read more (PubMed: 25056306) »

See all 7 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab28016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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