The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
Use at an assay dependent dilution.
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
Belongs to the HEXIM family.
The coiled-coil domain mediates oligomerization.
Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear.
Safronova OS et al. Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to promote gene-specific transcriptional repression. Nucleic Acids Res42:8954-69 (2014).
Read more (PubMed: 25056306) »
Natarajan M et al. Combinatorial signals from CD28 differentially regulate human immunodeficiency virus transcription in T cells. J Biol Chem285:17338-47 (2010).
Read more (PubMed: 20368329) »