The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
Ubiquitously expressed with higher expression in testis. HEXIM1 and HEXIM2 are differentially expressed.
Hexamethylene bis-acetamide-inducible protein 2 antibody
Hexamthylene bis-acetamide inducible 2 antibody
hexim 2 antibody
Protein HEXIM2 antibody
Immunoprecipitation - HEXIM2 antibody (ab112041)
Detection of HEXIM2 by Western Blot of Immunprecipitate.
ab112041 at 1µg/ml staining HEXIM2 in HeLa whole cell lysate immunoprecipitated using ab112041 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.