Overview

  • Product nameAnti-HEXO antibody
    See all HEXO primary antibodies
  • Description
    Rabbit polyclonal to HEXO
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: FAKKWINIRK SYGNFYKVPR SQTKLTIMLE KLGMDYDGRP HCGLDDSKNI, corresponding to C terminal amino acids 253-302 of Human HEXO

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
  • Sequence similaritiesContains 1 exonuclease domain.
    Contains 1 SAP domain.
  • DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.
  • Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3' 5' exonuclease ERI 1 antibody
    • 3' 5' exonuclease ERI1 antibody
    • 3' EXO antibody
    • 3' exoribonuclease antibody
    • 3' HEXO antibody
    • 3' histone mRNA exonuclease 1 antibody
    • 3''-5'' exonuclease eri1 antibody
    • 3''-5'' exoribonuclease 1 antibody
    • 3'EXO antibody
    • 3'HEXO antibody
    • ERI 1 antibody
    • Eri 1 homolog antibody
    • Eri-1 homolog antibody
    • eri1 antibody
    • Eri1 homolog antibody
    • ERI1_HUMAN antibody
    • HEXO antibody
    • Histone mRNA 3' end specific exonuclease antibody
    • Histone mRNA 3' end specific exoribonuclease antibody
    • Histone mRNA 3''-end-specific exoribonuclease antibody
    • Histone mRNA 3''-exonuclease 1 antibody
    • MGC35395 antibody
    • Protein 3''hExo antibody
    • THEX 1 antibody
    • THEX1 antibody
    • Three prime histone mRNA exonuclease 1 antibody
    see all

Anti-HEXO antibody images

  • All lanes : Anti-HEXO antibody (ab50959) at 2.5 µg/ml

    Lane 1 : Marker
    Lane 2 : HepG2 cell lysate

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 40 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-HEXO antibody (ab50959)

ab50959 has not yet been referenced specifically in any publications.

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