Overview

  • Product nameAnti-Hexokinase 1 antibody [4D7]
    See all Hexokinase 1 primary antibodies
  • Description
    Mouse monoclonal [4D7] to Hexokinase 1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-917 of Human Hexokinase 1, purified from E. coli.

  • Positive control
    • Human brain (cortex). Hep G2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab105213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 102 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityIsoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Involvement in diseaseHexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type
  • Sequence similaritiesBelongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • BB404130 antibody
    • Brain form hexokinase antibody
    • dea antibody
    • DrHXK1 antibody
    • EC 2.7.1.1 antibody
    • Glycolytic enzyme antibody
    • HEXOKIN antibody
    • hexokinase I antibody
    • Hexokinase PI antibody
    • Hexokinase type I antibody
    • Hexokinase, tumor isozyme antibody
    • Hexokinase-1 antibody
    • Hexokinase-A antibody
    • HK I antibody
    • HK1 antibody
    • HK1 tb antibody
    • Hk1-s antibody
    • HK1-ta antibody
    • HK1-tb antibody
    • HK1-tc antibody
    • HKD antibody
    • HKI antibody
    • HMSNR antibody
    • HXK1 antibody
    • HXK1_HUMAN antibody
    • im:7148527 antibody
    • mHk1-s antibody
    • wu:fc09d08 antibody
    • wu:fc16e02 antibody
    • wu:fc21e02 antibody
    • wu:fq14b11 antibody
    • zgc:55790 antibody
    • zgc:77618 antibody
    see all

Anti-Hexokinase 1 antibody [4D7] images

  • ab105213 at 5 µg/ml staining Hexokinase 1 in Human brain (cortex) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Hexokinase 1 antibody [4D7] (ab105213) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 102 kDa
  • ab105213 staining Hexokinase 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% NGS, 5% BSA in PBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in diluent) for 16 hours at 4°C. A Goat anti-mouse HRP IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-Hexokinase 1 antibody [4D7] (ab105213)

This product has been referenced in:
  • Lindvall ML  et al. A rat monoclonal antibody 4D7 produced by a hybridoma established from a yolk-sac tumor-bearing rat binds selectively to the stage-specific embryonic antigen SSEA-1. Int J Cancer 40:94-8 (1987). Read more (PubMed: 2885279) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (prostate)
Specification prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

Submitted Oct 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

Submitted Aug 09 2012

Thank you for your enquiry regarding ab105213.

Currently, we do not have any data regarding the reactivity of antibody ab105213 with mouse samples.
The immunogen was full-length human HK1 and at this time the epitope has not been mapped.<...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CLL)
Loading amount 30 µg
Specification CLL
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

Submitted May 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium citrate
Permeabilization No
Blocking step 10% NGS, 5%BSA in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

Submitted May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Treatment CoCl2 for 24h
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Hadas Pahima

Verified customer

Submitted Apr 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"