Overview

  • Product nameAnti-Hexokinase 1 antibody
    See all Hexokinase 1 primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Hexokinase 1.

  • Positive control
    • This antibody gave a positive signal in Hexokinase 1 Recombinant Protein as well as the following whole cell lysates: HeLa; Jurkat; MCF7; K562. his antibody gave a positive result in IHC in the following FFPE tissue: Humannormal lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab110529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Tissue specificityIsoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Involvement in diseaseHexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type
  • Sequence similaritiesBelongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • BB404130 antibody
    • Brain form hexokinase antibody
    • dea antibody
    • DrHXK1 antibody
    • EC 2.7.1.1 antibody
    • Glycolytic enzyme antibody
    • HEXOKIN antibody
    • hexokinase I antibody
    • Hexokinase PI antibody
    • Hexokinase type I antibody
    • Hexokinase, tumor isozyme antibody
    • Hexokinase-1 antibody
    • Hexokinase-A antibody
    • HK I antibody
    • HK1 antibody
    • HK1 tb antibody
    • Hk1-s antibody
    • HK1-ta antibody
    • HK1-tb antibody
    • HK1-tc antibody
    • HKD antibody
    • HKI antibody
    • HMSNR antibody
    • HXK1 antibody
    • HXK1_HUMAN antibody
    • im:7148527 antibody
    • mHk1-s antibody
    • wu:fc09d08 antibody
    • wu:fc16e02 antibody
    • wu:fc21e02 antibody
    • wu:fq14b11 antibody
    • zgc:55790 antibody
    • zgc:77618 antibody
    see all

Anti-Hexokinase 1 antibody images

  • All lanes : Anti-Hexokinase 1 antibody (ab110529) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
    Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Anti-Hexokinase 1 antibody (ab110529) at 1 µg/ml + Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
    Additional bands at : 147 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of Hexokinase 1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110529, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Hexokinase 1 antibody (ab110529)

ab110529 has not yet been referenced specifically in any publications.

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