Overview

  • Product name
    Anti-Hexokinase 1 antibody
    See all Hexokinase 1 primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Hexokinase 1.

  • Positive control
    • This antibody gave a positive signal in Hexokinase 1 Recombinant Protein and Human Testis tissue lysate as well as the following whole cell lysates: HeLa; MCF7; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab150013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

Target

  • Tissue specificity
    Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
  • Pathway
    Carbohydrate metabolism; hexose metabolism.
  • Involvement in disease
    Hexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type
  • Sequence similarities
    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • Domain
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localization
    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • BB404130 antibody
    • Brain form hexokinase antibody
    • dea antibody
    • DrHXK1 antibody
    • EC 2.7.1.1 antibody
    • Glycolytic enzyme antibody
    • HEXOKIN antibody
    • hexokinase I antibody
    • Hexokinase PI antibody
    • Hexokinase type I antibody
    • Hexokinase, tumor isozyme antibody
    • Hexokinase-1 antibody
    • Hexokinase-A antibody
    • HK I antibody
    • HK1 antibody
    • HK1 tb antibody
    • Hk1-s antibody
    • HK1-ta antibody
    • HK1-tb antibody
    • HK1-tc antibody
    • HKD antibody
    • HKI antibody
    • HMSNR antibody
    • HXK1 antibody
    • HXK1_HUMAN antibody
    • im:7148527 antibody
    • mHk1-s antibody
    • wu:fc09d08 antibody
    • wu:fc16e02 antibody
    • wu:fc21e02 antibody
    • wu:fq14b11 antibody
    • zgc:55790 antibody
    • zgc:77618 antibody
    see all

Images

  • All lanes : Anti-Hexokinase 1 antibody (ab150013) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa
    Additional bands at: 46 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • Anti-Hexokinase 1 antibody (ab150013) at 1/1 dilution + Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa


    Exposure time: 1 minute

References

ab150013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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