This antibody gave a positive signal in Hexokinase 1 Recombinant Protein and Human Testis tissue lysate as well as the following whole cell lysates: HeLa; MCF7; K562.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
Carbohydrate metabolism; hexose metabolism.
Involvement in disease
Hexokinase deficiency Neuropathy, hereditary motor and sensory, Russe type
Belongs to the hexokinase family. Contains 2 hexokinase domains.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.