The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
1/50 - 1/100.
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Carbohydrate metabolism; hexose metabolism.
Belongs to the hexokinase family. Contains 2 hexokinase domains.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
Anti-Hexokinase II antibody (ab37593) at 1/500 dilution + A375 cell lysate
Predicted band size : 102 kDa Observed band size : 102 kDa Additional bands at : 30 kDa,40 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hexokinase II antibody (ab37593)
ab37593, at 1/100 dilution, staining human hepatocarcinoma tissue by immunohistochemistry.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.