Overview

  • Product nameAnti-Hexokinase II antibody
    See all Hexokinase II primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase II
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificityPredominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Sequence similaritiesBelongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

Anti-Hexokinase II antibody images

  • Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ab76358)
  • Anti-Hexokinase II antibody (ab76358) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 88 kDa (why is the actual band size different from the predicted?)
    Gel concentration 6-18%
  • ab7638 at 5 µg/ml staining Hexokinase II in Human skeletal muscle by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Hexokinase II antibody (ab76358)

ab76358 has not yet been referenced specifically in any publications.

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