Purification notesab78259 is partially purified rabbit serum. Antibodies reactive with the Type I isozyme were present in the original antiserum; these were removed by affinity chromatography on a column of immobilized Type I isozyme, and the affinity-depleted serum restored to original volume.
Sequence similaritiesBelongs to the hexokinase family. Contains 2 hexokinase domains.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.