Anti-Hexokinase II antibody - N-terminal (ab71725)

Overview

  • Product nameAnti-Hexokinase II antibody - N-terminal
    See all Hexokinase II primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase II - N-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human Hexokinase II conjugated to KLH

  • Positive control
    • A375 cell lysate and human skeletal muscle tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

Target

  • Tissue specificityPredominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Sequence similaritiesBelongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

Anti-Hexokinase II antibody - N-terminal images

  • Anti-Hexokinase II antibody - N-terminal (ab71725) at 1/100 dilution + A375 cell lysate at 12.5 µg

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab71725 at 1/50 dilution, staining Hexokinase II in human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-Hexokinase II antibody - N-terminal (ab71725)

ab71725 has not yet been referenced specifically in any publications.

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