Overview

  • Product name
    Anti-Hexokinase Type III antibody
    See all Hexokinase Type III primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase Type III
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 587-786 of Human Hexokinase Type III (UniProt P52790).

  • Positive control
    • Hela whole cell lysate; A549 and HCT116 cell lysates; Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab126217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
ICC/IF 1/100 - 1/500.

Target

Images

  • Anti-Hexokinase Type III antibody (ab126217) at 1/1000 dilution + Hela whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • ab126217, at 1/500 dilution, staining Hexokinase Type III in methanol fixed Hela cells by Immunofluorescence.
    The image in the lower panel was costained with Hoechst 33342.

References

This product has been referenced in:
  • Kuang R  et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . Read more (PubMed: 28138554) »
  • Cao Y  et al. Metabolic reprogramming towards aerobic glycolysis correlates with greater proliferative ability and resistance to metabolic inhibition in CD8 versus CD4 T cells. PLoS One 9:e104104 (2014). WB ; Mouse . Read more (PubMed: 25090630) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (rat heart tissue)
Antigen retrieval step
Other
Permeabilization
Yes - methanol
Specification
rat heart tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Username

Dr. Barbara Elsnicová

Verified customer

Submitted Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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