Anti-Hexokinase Type III antibody (ab60225)

Overview

  • Product name
    Anti-Hexokinase Type III antibody
    See all Hexokinase Type III primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase Type III
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the N terminal region of human Hexokinase Type III conjugated to KLH

  • Positive control
    • HeLa cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ELISA 1/1000.

Target

  • Pathway
    Carbohydrate metabolism; hexose metabolism.
  • Sequence similarities
    Belongs to the hexokinase family.
  • Domain
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Images

  • Anti-Hexokinase Type III antibody (ab60225) at 1/40 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 99 kDa
    Observed band size : 99 kDa

References

ab60225 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply.

We have not tested ab60225 with mouse tissue but based on sequence homology (85%) there is a good chance that it will work.

Please let me know if there is anything else I can help you with.

Thank you for contacting Abcam.

The primary antibody concentration used for the western blot image was indeed 1/40, but we recommend 1/100-1/500, as the signal from the band is very strong and so therefore you should be able to get a good sig...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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