Anti-Hexokinase Type III antibody (ab60225)

Overview

  • Product name
    Anti-Hexokinase Type III antibody
    See all Hexokinase Type III primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase Type III
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the N terminal region of human Hexokinase Type III conjugated to KLH

  • Positive control
    • HeLa cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ELISA 1/1000.

Target

  • Pathway
    Carbohydrate metabolism; hexose metabolism.
  • Sequence similarities
    Belongs to the hexokinase family.
  • Domain
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Anti-Hexokinase Type III antibody images

  • Anti-Hexokinase Type III antibody (ab60225) at 1/40 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 99 kDa
    Observed band size : 99 kDa

References for Anti-Hexokinase Type III antibody (ab60225)

ab60225 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

We have not tested ab60225 with mouse tissue but based on sequence homology (85%) there is a good chance that it will work.

Please let me know if there is anything else I can help you with.

Thank you for contacting Abcam.

The primary antibody concentration used for the western blot image was indeed 1/40, but we recommend 1/100-1/500, as the signal from the band is very strong and so therefore you should be able to get a good sig...

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