Anti-Hexokinase Type III antibody (ab91097)


  • Product nameAnti-Hexokinase Type III antibody
    See all Hexokinase Type III primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase Type III
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Hexokinase Type III .

  • Positive control
    • This antibody gave a positive signal in both Human liver and spleen tissue lysates.


Associated products


Our Abpromise guarantee covers the use of ab91097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).


  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Sequence similaritiesBelongs to the hexokinase family.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Anti-Hexokinase Type III antibody images

  • All lanes : Anti-Hexokinase Type III antibody (ab91097) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 175 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab91097 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91097 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Hexokinase Type III antibody (ab91097)

ab91097 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91097.
Please use the links above to contact us or submit feedback about this product.