Anti-Hexokinase Type III antibody (ab91097)


  • Product name
    Anti-Hexokinase Type III antibody
    See all Hexokinase Type III primary antibodies
  • Description
    Rabbit polyclonal to Hexokinase Type III
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Hexokinase Type III .

  • Positive control
    • This antibody gave a positive signal in both Human liver and spleen tissue lysates.



Our Abpromise guarantee covers the use of ab91097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).


  • Pathway
    Carbohydrate metabolism; hexose metabolism.
  • Sequence similarities
    Belongs to the hexokinase family.
  • Domain
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Anti-Hexokinase Type III antibody images

  • All lanes : Anti-Hexokinase Type III antibody (ab91097) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 175 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab91097 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91097 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Hexokinase Type III antibody (ab91097)

ab91097 has not yet been referenced specifically in any publications.

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