This antibody gave a positive signal in both Human liver and spleen tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Sequence similaritiesBelongs to the hexokinase family.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
Western blot - Hexokinase Type III antibody (ab91097)
All lanes : Anti-Hexokinase Type III antibody (ab91097) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 99 kDa Observed band size : 99 kDa Additional bands at : 175 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase Type III antibody (ab91097)
ICC/IF image of ab91097 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91097 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Hexokinase Type III antibody (ab91097)
has not yet been referenced specifically in any publications.
Publishing research using ab91097? Please let us know so that we can cite the reference in this datasheet.
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