Overview

  • Product name
  • Description
    Rabbit polyclonal to Hey L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 45-184 of Human Hey L.

  • Positive control
    • A431, H1299, HeLa, HepG2 whole cell lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab153900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb33 antibody
    • Class B basic helix loop helix protein 33 antibody
    • Class B basic helix-loop-helix protein 33 antibody
    • Hairy enhancer of split related with YRPW motif like antibody
    • Hairy related transcription factor 3 antibody
    • Hairy-related transcription factor 3 antibody
    • Hairy/enhancer of split related with YRPW motif 3 antibody
    • Hairy/enhancer of split related with YRPW motif like antibody
    • Hairy/enhancer of split related with YRPW motif like protein antibody
    • Hairy/enhancer-of-split related with YRPW motif-like protein antibody
    • HEY like protein antibody
    • HEY3 antibody
    • heyl antibody
    • HEYL protein antibody
    • HEYL_HUMAN antibody
    • hHeyL antibody
    • hHRT3 antibody
    • HRT 3 antibody
    • HRT-3 antibody
    • HRT3 antibody
    • MGC12623 antibody
    see all

Images

  • Anti-Hey L antibody (ab153900) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Hey L with ab153900 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab153900 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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