Overview

  • Product nameAnti-Hey L antibody
    See all Hey L primary antibodies
  • Description
    Rabbit polyclonal to Hey L
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MKRPKEPSGS DGESDGPIDV GQEGQLSQMA RPLSTPSSSQ MQARKKRRGI, corresponding to N terminal amino acids 1-50 of Human Hey L

  • Positive control
    • Foetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab26138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
ELISA 1/312500.

Target

  • FunctionDownstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similaritiesBelongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb33 antibody
    • Class B basic helix loop helix protein 33 antibody
    • Class B basic helix-loop-helix protein 33 antibody
    • Hairy enhancer of split related with YRPW motif like antibody
    • Hairy related transcription factor 3 antibody
    • Hairy-related transcription factor 3 antibody
    • Hairy/enhancer of split related with YRPW motif 3 antibody
    • Hairy/enhancer of split related with YRPW motif like antibody
    • Hairy/enhancer of split related with YRPW motif like protein antibody
    • Hairy/enhancer-of-split related with YRPW motif-like protein antibody
    • HEY like protein antibody
    • HEY3 antibody
    • heyl antibody
    • HEYL protein antibody
    • HEYL_HUMAN antibody
    • hHeyL antibody
    • hHRT3 antibody
    • HRT 3 antibody
    • HRT-3 antibody
    • HRT3 antibody
    • MGC12623 antibody
    see all

Anti-Hey L antibody images

  • Anti-Hey L antibody (ab26138) at 1.25 µg/ml + Foetal muscle lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 35 kDa
  • Immunofluorescence analysis of HUVEC cells, staining Hey L with ab26138.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Hey L antibody (ab26138)

This product has been referenced in:
  • Morrow D  et al. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol 29:1112-8 (2009). Read more (PubMed: 19407245) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS1 cell line)
Specification COS1 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEK cells)
Specification HUVEK cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2012

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit polyclonal to Hey L (ab26138) has been tested and shown to work in ELISA and western blotting. To date it has not been tested for its a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"