The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Belongs to the HEY family. Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.
Class B basic helix loop helix protein 33 antibody
Class B basic helix-loop-helix protein 33 antibody
Hairy enhancer of split related with YRPW motif like antibody
Hairy related transcription factor 3 antibody
Hairy-related transcription factor 3 antibody
Hairy/enhancer of split related with YRPW motif 3 antibody
Hairy/enhancer of split related with YRPW motif like antibody
Hairy/enhancer of split related with YRPW motif like protein antibody
Hairy/enhancer-of-split related with YRPW motif-like protein antibody
HEY like protein antibody
HEYL protein antibody
HRT 3 antibody
Western blot - Anti-Hey L antibody (ab26138)
Anti-Hey L antibody (ab26138) at 1.25 µg/ml + Foetal muscle lysate
Secondary HRP conjugated anti-Rabbit IgG
Predicted band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Hey L antibody (ab26138)This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of HUVEC cells, staining Hey L with ab26138.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.