Overview

  • Product name
  • Description
    Rabbit polyclonal to Hey L
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    STPSSSQ MQARKKH

    , corresponding to amino acids 34-47 of Human Hey L

  • General notes

    Reconstitution Notes:

    To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

    Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL.  Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal.


    The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity.

Properties

Applications

Our Abpromise guarantee covers the use of ab78048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/1000.
ICC/IF Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 35 kDa.

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb33 antibody
    • Class B basic helix loop helix protein 33 antibody
    • Class B basic helix-loop-helix protein 33 antibody
    • Hairy enhancer of split related with YRPW motif like antibody
    • Hairy related transcription factor 3 antibody
    • Hairy-related transcription factor 3 antibody
    • Hairy/enhancer of split related with YRPW motif 3 antibody
    • Hairy/enhancer of split related with YRPW motif like antibody
    • Hairy/enhancer of split related with YRPW motif like protein antibody
    • Hairy/enhancer-of-split related with YRPW motif-like protein antibody
    • HEY like protein antibody
    • HEY3 antibody
    • heyl antibody
    • HEYL protein antibody
    • HEYL_HUMAN antibody
    • hHeyL antibody
    • hHRT3 antibody
    • HRT 3 antibody
    • HRT-3 antibody
    • HRT3 antibody
    • MGC12623 antibody
    see all

Anti-Hey L antibody images

  • Immunofluorescence analysis of HUVEC cells, staining Hey L with ab78048.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

  • ab14404 staining Hey L in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Hey L antibody (ab78048)

ab78048 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEK cells)
Specification
HUVEK cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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