Overview

  • Product nameAnti-Hey L antibody
    See all Hey L primary antibodies
  • Description
    Rabbit polyclonal to Hey L
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    STPSSSQ MQARKKH

    , corresponding to amino acids 34-47 of Human Hey L

Properties

  • FormLyophilised:To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 50% Ammonium sulphate, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200 - 1/1000.
ICC/IF Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 35 kDa.

Target

  • FunctionDownstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similaritiesBelongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb33 antibody
    • Class B basic helix loop helix protein 33 antibody
    • Class B basic helix-loop-helix protein 33 antibody
    • Hairy enhancer of split related with YRPW motif like antibody
    • Hairy related transcription factor 3 antibody
    • Hairy-related transcription factor 3 antibody
    • Hairy/enhancer of split related with YRPW motif 3 antibody
    • Hairy/enhancer of split related with YRPW motif like antibody
    • Hairy/enhancer of split related with YRPW motif like protein antibody
    • Hairy/enhancer-of-split related with YRPW motif-like protein antibody
    • HEY like protein antibody
    • HEY3 antibody
    • heyl antibody
    • HEYL protein antibody
    • HEYL_HUMAN antibody
    • hHeyL antibody
    • hHRT3 antibody
    • HRT 3 antibody
    • HRT-3 antibody
    • HRT3 antibody
    • MGC12623 antibody
    see all

Anti-Hey L antibody images

  • Immunofluorescence analysis of HUVEC cells, staining Hey L with ab78048.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

  • ab14404 staining Hey L in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Hey L antibody (ab78048)

ab78048 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS1 cell line)
Specification COS1 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEK cells)
Specification HUVEK cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"