Overview

  • Product nameAnti-HEY1 antibody
    See all HEY1 primary antibodies
  • Description
    Rabbit polyclonal to HEY1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human HEY1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9Y5J3

  • Positive control
    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab111723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • FunctionDownstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Tissue specificityExpressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • Sequence similaritiesBelongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all

Anti-HEY1 antibody images

  • Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-HEY1 antibody (ab111723)

ab111723 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111723.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"