Anti-HEY1 antibody (ab154077)
Key features and details
- Rabbit polyclonal to HEY1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HEY1 antibody
See all HEY1 primary antibodies -
Description
Rabbit polyclonal to HEY1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Recombinant fragment corresponding to a region within amino acids 12-304 of Human HEY1.
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Positive control
- A549, H1299, HCT116 and MCF-7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/100 - 1/2000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/100 - 1/2000. |
Target
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Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. -
Tissue specificity
Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs. -
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 408005 Cow
- Entrez Gene: 403420 Dog
- Entrez Gene: 23462 Human
- Entrez Gene: 15213 Mouse
- Entrez Gene: 155437 Rat
- Omim: 602953 Human
- SwissProt: Q2KIN4 Cow
- SwissProt: Q9TSZ2 Dog
see all -
Alternative names
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
see all
Images
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All lanes : Anti-HEY1 antibody (ab154077) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
ab154077 staining HEY1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/2000) (green). Alpha tubulin stained red and nuclei stained blue
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ab154077 staining HEY1 in H1299 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/1000) (green). Nuclei were stained blue with Hoechst 33342
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab154077 has been referenced in 19 publications.
- Zhang S et al. GIT1 protects against breast cancer growth through negative regulation of Notch. Nat Commun 13:1537 (2022). PubMed: 35318302
- Chen Y et al. Galectin-3 enhances trastuzumab resistance by regulating cancer malignancy and stemness in HER2-positive breast cancer cells. Thorac Cancer 13:1961-1973 (2022). PubMed: 35599381
- Cui X et al. HOCI Probe CPP Induces the Differentiation of Human Dermal Fibroblasts into Vascular Endothelial Cells through PHD2/HIF-1α/HEY1 Signaling Pathway. Cells 11:N/A (2022). PubMed: 36231088
- Nishad R et al. Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria. Cell Death Dis 12:342 (2021). PubMed: 33795655
- Tao ZS et al. Intermittent administration sodium valproate has a protective effect on bone health in ovariectomized rats. Eur J Pharmacol 906:174268 (2021). PubMed: 34166702