Overview

  • Product name
  • Description
    Rabbit polyclonal to HEY1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 12-304 of Human HEY1.

  • Positive control
    • A549, H1299, HCT116 and MCF-7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab154077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
ICC/IF 1/100 - 1/2000.

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Tissue specificity
    Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all

Images

  • All lanes : Anti-HEY1 antibody (ab154077) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MCF-7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS PAGE
  • ab154077 staining HEY1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/2000) (green). Alpha tubulin stained red and nuclei stained blue

  • ab154077 staining HEY1 in H1299 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/1000) (green). Nuclei were stained blue with Hoechst 33342

References

This product has been referenced in:
  • Wang T  et al. Fibulin-4 is associated with prognosis of endometrial cancer patients and inhibits cancer cell invasion and metastasis via Wnt/ß-catenin signaling pathway. Oncotarget 8:18991-19012 (2017). WB ; Human . Read more (PubMed: 28177909) »
  • Lu W  et al. Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2aImproves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model. Stem Cells Int 2017:3794817 (2017). Read more (PubMed: 29238372) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6)
Permeabilization
Yes - Triton-X (0.1% in PBS)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Submitted Jan 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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