Overview

  • Product name
  • Description
    Rabbit polyclonal to HEY2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse HEY2/ Hry2.

    (Peptide available as ab38053.)

  • Positive control
    • ab25404 gave a positive signal in Mouse Heart and Skeletal Muscle Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab25404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb32 antibody
    • Cardiovascular helix loop helix factor 1 antibody
    • Cardiovascular helix-loop-helix factor 1 antibody
    • CHF1 antibody
    • Class B basic helix-loop-helix protein 32 antibody
    • GRIDLOCK antibody
    • GRL antibody
    • Hairy and enhancer of split related 2 antibody
    • Hairy and enhancer of split-related protein 2 antibody
    • Hairy related transcription factor 2 antibody
    • Hairy-related transcription factor 2 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
    • hCHF1 antibody
    • HERP antibody
    • HERP1 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-2 antibody
    • HESR2 antibody
    • hey2 antibody
    • HEY2_HUMAN antibody
    • hHRT2 antibody
    • HRT-2 antibody
    • HRT2 antibody
    • MGC10720 antibody
    • Protein gridlock homolog antibody
    see all

Images

  • All lanes : Anti-HEY2 antibody (ab25404) at 2 µg/ml

    Lane 1 : Heart (Mouse)Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Redmond EM  et al. Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling. PLoS One 9:e84122 (2014). WB ; Mouse . Read more (PubMed: 24416200) »
  • Hu GH  et al. Delta-like ligand 4 (Dll4) predicts the prognosis of clear cell renal cell carcinoma, and anti-Dll4 suppresses tumor growth in vivo. Int J Clin Exp Pathol 7:2143-52 (2014). WB . Read more (PubMed: 24966922) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEY2 overexpressing human pluripotent stem cell)
Permeabilization
Yes - saponin
Specification
HEY2 overexpressing human pluripotent stem cell
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 12 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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