• Product nameAnti-HEY2 antibody
    See all HEY2 primary antibodies
  • Description
    Rabbit polyclonal to HEY2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse HEY2/ Hry2.

    (Peptide available as ab38053.)

  • Positive control
    • ab25404 gave a positive signal in Mouse Heart and Skeletal Muscle Tissue Lysates.



Our Abpromise guarantee covers the use of ab25404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).


  • FunctionDownstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similaritiesBelongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb32 antibody
    • Cardiovascular helix loop helix factor 1 antibody
    • Cardiovascular helix-loop-helix factor 1 antibody
    • CHF1 antibody
    • Class B basic helix-loop-helix protein 32 antibody
    • GRIDLOCK antibody
    • GRL antibody
    • Hairy and enhancer of split related 2 antibody
    • Hairy and enhancer of split-related protein 2 antibody
    • Hairy related transcription factor 2 antibody
    • Hairy-related transcription factor 2 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
    • hCHF1 antibody
    • HERP antibody
    • HERP1 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-2 antibody
    • HESR2 antibody
    • hey2 antibody
    • HEY2_HUMAN antibody
    • hHRT2 antibody
    • HRT-2 antibody
    • HRT2 antibody
    • MGC10720 antibody
    • Protein gridlock homolog antibody
    see all

Anti-HEY2 antibody images

  • All lanes : Anti-HEY2 antibody (ab25404) at 2 µg/ml

    Lane 1 : Heart (Mouse)Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HEY2 antibody (ab25404)

This product has been referenced in:
  • Redmond EM  et al. Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling. PLoS One 9:e84122 (2014). WB ; Mouse . Read more (PubMed: 24416200) »
  • Hu GH  et al. Delta-like ligand 4 (Dll4) predicts the prognosis of clear cell renal cell carcinoma, and anti-Dll4 suppresses tumor growth in vivo. Int J Clin Exp Pathol 7:2143-52 (2014). WB . Read more (PubMed: 24966922) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEY2 overexpressing human pluripotent stem cell)
Permeabilization Yes - saponin
Specification HEY2 overexpressing human pluripotent stem cell
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 12 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 06 2008