Anti-HFE antibody (ab55182)

Overview

  • Product nameAnti-HFE antibody
    See all HFE primary antibodies
  • Description
    Mouse monoclonal to HFE
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ Q, corresponding to amino acids 115-206 of Human HFE

Properties

Applications

Our Abpromise guarantee covers the use of ab55182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
    • Tissue specificityExpressed in all tissues tested except brain.
    • Involvement in diseaseDefects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
      Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
      Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • Sequence similaritiesBelongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • dJ221C16.10.1 antibody
      • Hemochromatosis antibody
      • Hemochromatosis protein antibody
      • Hereditary hemochromatosis protein antibody
      • Hereditary hemochromatosis protein HLA H antibody
      • HFE 1 antibody
      • HFE antibody
      • HFE_HUMAN antibody
      • HFE1 antibody
      • HH antibody
      • High Fe antibody
      • HLA H antibody
      • HLA-H antibody
      • HLAH antibody
      • MGC:150812 antibody
      • MGC10379 antibody
      • MGC103790 antibody
      • MHC class I like protein HFE antibody
      • MVCD7 antibody
      • TFQTL2 antibody
      see all

    Anti-HFE antibody images

    • ICC/IF image of HFE staining (ab55182) in human blood film. The sections were incubated in 0.3% Triton X with 0.1M PBS to permeabilise the cells and in 10% serum for 30 minutes at RT to block non-specific protein-protein interactions. The sections were then incubated with ab55182 (1:500) for 4 hrs at RT, followed by Alexa 568 conjugated secondary antibody. Red staining of the cell membrane was observed. Macrophages were stained using a CD68 primary and Alexa Fluor 488 conjugated secondary antibody. The nucleus was visualised using Hoechst.

      See Abreview

    • Western blot against tagged recombinant protein immunogen using ab55182 HFE antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
    • ab55182 at 10 ug/ml staining HFE in human Hela cells by Immunocytochemistry/ Immunofluorescence.

    References for Anti-HFE antibody (ab55182)

    ab55182 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2)
    Loading amount 50 µg
    Specification HepG2
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 06 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 28 2012

    Thank you very much for your interest in ab102592.

    To our knowledge, ab102592 has not been tested in rat samples. Therefore, I can offer a discount off a future purchase if you buy ab102592 now, test it in rat and submit feedback to us in the ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2)
    Loading amount 50 µg
    Specification HepG2
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Blood film)
    Specification Blood film
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX in 0.1M PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"