Overview

  • Product name
  • Description
    Rabbit polyclonal to HFH4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HFH4.

  • Positive control
    • This antibody gave a positive signal in A549 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    May play an important role in cell fate determination during lung development and in spermatogenesis.
  • Tissue specificity
    Testis, oviduct, lung and brain cortex.
  • Involvement in disease
    Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Developmental stage
    Expressed in developing lung, kidney and central nervous system.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKHL13 antibody
    • Forkhead box J1 antibody
    • Forkhead box protein J1 antibody
    • Forkhead Homologue 4 antibody
    • Forkhead related protein FKHL13 antibody
    • Forkhead-related protein FKHL13 antibody
    • Fox J1 antibody
    • Foxj1 antibody
    • FOXJ1_HUMAN antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 4 antibody
    • HFH 4 antibody
    • HFH-4 antibody
    • HFH4 antibody
    • HNF 3/forkhead homolog 4 antibody
    • MGC35202 antibody
    see all

Images

  • All lanes : Anti-HFH4 antibody (ab108452) at 1/250 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1/250 dilution

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

References

ab108452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab108452.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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