Anti-FoxJ1 antibody [CL3989] (ab220028)
Key features and details
- Mouse monoclonal [CL3989] to FoxJ1
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FoxJ1 antibody [CL3989]
See all FoxJ1 primary antibodies -
Description
Mouse monoclonal [CL3989] to FoxJ1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human FoxJ1 aa 150-350.
Database link: Q92949 -
Epitope
Binds to an epitope located within the peptide sequence EPGKGGFWRI (aa 188-197) as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human fallopian tube and endometrium tissues. ICC: BEWO cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3989 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
May play an important role in cell fate determination during lung development and in spermatogenesis. -
Tissue specificity
Testis, oviduct, lung and brain cortex. -
Involvement in disease
Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Developmental stage
Expressed in developing lung, kidney and central nervous system. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2302 Human
- Entrez Gene: 15223 Mouse
- Entrez Gene: 116557 Rat
- Omim: 602291 Human
- SwissProt: Q92949 Human
- SwissProt: Q61660 Mouse
- SwissProt: Q63247 Rat
- Unigene: 651204 Human
see all -
Alternative names
- FKHL13 antibody
- Forkhead box J1 antibody
- Forkhead box protein J1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence analysis of BEWO cells labeling FoxJ1 with ab220028 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab220028 has been referenced in 1 publication.
- Liu J et al. Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking. PLoS Biol 17:e3000203 (2019). PubMed: 31430272