For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
Our Abpromise guarantee covers the use of ab10678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml.
Use at 0.5 - 1 µg/ml to detect <1.2 ng/well of rhHGF.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83.1 kDa.
Use at 1 - 2 µg/ml to detect rhHGF at 50 ng/lane under non-reducing conditions.
|Neutralising||Use a concentration of 0.01 - 0.5 µg/ml.
Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells were added to each well and incubated for 48 hours at 37°C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA was measured.