Overview

  • Product nameAnti-HGF antibody [24612.111]
    See all HGF primary antibodies
  • Description
    Mouse monoclonal [24612.111] to HGF
  • SpecificityThe product shows no cross reactivity with other tested cytokines using indirect ELISA.
  • Tested applicationsSuitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.

  • Positive control
  • General notesEndotoxin: <5 ng/vial by LAL method.


    Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThe antibody is purified using Protein A affinity chromatography.
  • Primary antibody notesHepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
  • ClonalityMonoclonal
  • Clone number24612.111
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 - 1 µg/ml.

Use at 0.5 - 1 µg/ml to detect <1.2 ng/well of rhHGF.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83.1 kDa.

Use at 1 - 2 µg/ml to detect rhHGF at 50 ng/lane under non-reducing conditions.

Neutralising Use a concentration of 0.01 - 0.5 µg/ml.

Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells were added to each well and incubated for 48 hours at 37°C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA was measured.

Target

  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNB39 antibody
    • F TCF antibody
    • Fibroblast derived tumor cytotoxic factor antibody
    • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
    • Hepatocyte growth factor antibody
    • Hepatocyte growth factor beta chain antibody
    • Hepatocyte growth factor precursor antibody
    • Hepatopoietin A antibody
    • Hepatopoietin-A antibody
    • Hgf antibody
    • HGF_HUMAN antibody
    • HGFB antibody
    • HPTA antibody
    • Lung fibroblast derived mitogen antibody
    • OTTHUMP00000161349 antibody
    • OTTHUMP00000206710 antibody
    • OTTHUMP00000206711 antibody
    • OTTHUMP00000206712 antibody
    • OTTHUMP00000206713 antibody
    • OTTHUMP00000206730 antibody
    • Scatter factor antibody
    • SF antibody
    see all

References for Anti-HGF antibody [24612.111] (ab10678)

This product has been referenced in:
  • Li M  et al. Hepatocyte growth factor and basic fibroblast growth factor regulate atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease via the mitogen-activated protein kinase signaling pathway. Exp Ther Med 6:1121-1126 (2013). Human . Read more (PubMed: 24223632) »
  • Casbas-Hernandez P  et al. Role of HGF in epithelial-stromal cell interactions during progression from benign breast disease to ductal carcinoma in situ. Breast Cancer Res 15:R82 (2013). Read more (PubMed: 24025166) »

See all 3 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf. Die Referenznummer Ihrer Gutschrift ist xxx. Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutschrift für Sie auszus...

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We don't have a protocol for use of this antibody in neutralisation studies. Here is a useful reference regarding that application: Rubin, J., et al., Proc. Natl. Acad. Sci. USA, 88, 415 (1991). I hope this is helpful. Please contact us again i...

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Thank you for contacting us. I am sorry to hear that yo are having problems with this antibody. I am checking with the lab for additional information besides this: This monoclonal Anti-Human HGF was tested for its ability to neutral...

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Thanks for your patience. For ab10678, ab10679, and ab10680, I cannot obtain any more information other than what is on our online datasheets. For ab4260, the originator has informed me that the epitope has not been determined for this monoclonal antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"