Overview

  • Product nameAnti-HGF antibody
    See all HGF primary antibodies
  • Description
    Goat polyclonal to HGF
  • SpecificityThe product shows no cross reactivity with other tested cytokines using indirect ELISA.
  • Tested applicationsSuitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant, human hepatocyte growth factor (rhHGF), expressed in the insect cell line Sf 21.

  • Positive control
  • General notesThe endotoxin level is less than 10 ng/vial (LAL method).


    Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupfer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe product is purified by Protein G affinity chromatography.
  • Primary antibody notesHepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupfer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

Use at 0.5 µg/ml, this detects 0.31ng/well of rhHGF

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83.1 kDa.

Use at a concentration of 1 µg/ml, this detects rhHGF at 50 ng/lane under both reducing and non-reducing conditions.

Neutralising Use a concentration of 1 - 16 µg/ml.

ab10679 was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF, which is present at five times its own EC50 (the concentration of rhHGF producing a one-half maximal bioactivity without antibody). In this bioassay, rhHGF was pre-incubated with various dilutions of the antibody for 1hr at 37 °C in a 96 well plate. Then, 4MBr-5 cells were added to each well to give a final concentration of 3 x 105 cells/ml in 0.1ml containing 100ng/ml rhHGF. This was incubated for 48hrs at 37 °C in a 5% CO2 humidified incubator and then pulsed for 4hrs with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorp

Target

  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNB39 antibody
    • F TCF antibody
    • Fibroblast derived tumor cytotoxic factor antibody
    • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
    • Hepatocyte growth factor antibody
    • Hepatocyte growth factor beta chain antibody
    • Hepatocyte growth factor precursor antibody
    • Hepatopoietin A antibody
    • Hepatopoietin-A antibody
    • Hgf antibody
    • HGF_HUMAN antibody
    • HGFB antibody
    • HPTA antibody
    • Lung fibroblast derived mitogen antibody
    • OTTHUMP00000161349 antibody
    • OTTHUMP00000206710 antibody
    • OTTHUMP00000206711 antibody
    • OTTHUMP00000206712 antibody
    • OTTHUMP00000206713 antibody
    • OTTHUMP00000206730 antibody
    • Scatter factor antibody
    • SF antibody
    see all

References for Anti-HGF antibody (ab10679)

This product has been referenced in:
  • Díaz-Coránguez M  et al. Transmigration of neural stem cells across the blood brain barrier induced by glioma cells. PLoS One 8:e60655 (2013). Neutralising ; Human . Read more (PubMed: 23637756) »
  • Kimoto M  et al. Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci 53:7583-9 (2012). Neutralising ; Human . Read more (PubMed: 23081981) »

See all 3 Publications for this product

Product Wall

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I have been in touch with the lab regarding your request. The antibody, ab10679 Anti-HGF is tested in western blotting for detection of human recombinant HGF. We do offer recombinant full length Human HGF, ab10...

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For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for your enquiry. I'm very sorry to hear you are having problems with this antibody. I understand your problem and agree that the molecular weight is very similar to that of the alpha chain (which is 31.5-34.5 kDa)of HGF. As it is linked to ...

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Thanks for your patience. For ab10678, ab10679, and ab10680, I cannot obtain any more information other than what is on our online datasheets. For ab4260, the originator has informed me that the epitope has not been determined for this monoclonal antib...

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Thank you for your email. When we have images available for an antibody, we place the image on the online datasheet. Unfortunately, we currently do not have any images available for ab10679. I'm afraid that all the information that we have at this time...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. Ab10679 was tested for application in ELISA and WB and cross-reacts with human. To our knowledge, customized protocols are not requ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"