Our Abpromise guarantee covers the use of ab24865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. (see Abreview)
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab10829 as Capture.


  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNB39 antibody
    • F TCF antibody
    • Fibroblast derived tumor cytotoxic factor antibody
    • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
    • Hepatocyte growth factor antibody
    • Hepatocyte growth factor beta chain antibody
    • Hepatocyte growth factor precursor antibody
    • Hepatopoietin A antibody
    • Hepatopoietin-A antibody
    • Hgf antibody
    • HGF_HUMAN antibody
    • HGFB antibody
    • HPTA antibody
    • Lung fibroblast derived mitogen antibody
    • OTTHUMP00000161349 antibody
    • OTTHUMP00000206710 antibody
    • OTTHUMP00000206711 antibody
    • OTTHUMP00000206712 antibody
    • OTTHUMP00000206713 antibody
    • OTTHUMP00000206730 antibody
    • Scatter factor antibody
    • SF antibody
    see all

Anti-HGF antibody images

  • Standard Curve for HGF (Analyte: ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.
  • Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-HGF antibody (ab24865)

This product has been referenced in:
  • Cohen ME  et al. Signaling through three chemokine receptors triggers the migration of transplanted neural precursor cells in a model of multiple sclerosis. Stem Cell Res 13:227-39 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25086214) »
  • Goldoni S  et al. Decorin is a novel antagonistic ligand of the Met receptor. J Cell Biol 185:743-54 (2009). WB ; Human . Read more (PubMed: 19433454) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil,cerebellum,fetal liver,normal breast,fetal)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
Permeabilization Yes - 0.05%Tween20 in PBS
Specification Tonsil,cerebellum,fetal liver,normal breast,fetal
Blocking step sea block (fisher#37527) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2017

Thank you for contacting Abcam.

The antibody was generated against the active recombinant protein of HGF, so it should also recognize the inactive precursor form. However we do not have the possibility to check it.

Also, there is a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human mesenchymal stem cells transduced with Adv-H)
Specification Human mesenchymal stem cells transduced with Adv-H
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Mr. Hao Wu

Verified customer

Submitted Dec 03 2010