Overview

  • Product nameHGF Mouse ELISA KitSee all HGF kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.4 ng/ml
  • Range
    0.614 ng/ml - 60 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111.8 98% - 123%
    Serum 111.4 98% - 129%
    Plasma 105.8 80% - 120%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionHGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
  • Involvement in diseaseDefects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Target information above from: UniProt accession P14210 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepapoietin A
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A scatter factor
    • Hepatocyte growth factor beta chain
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • Scatter factor
    • SF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

HGF Mouse ELISA Kit images

  • Representative Standard Curve using ab100686

  • Representative Standard Curve using ab100686

Protocols

References for HGF Mouse ELISA Kit (ab100686)

ab100686 has not yet been referenced specifically in any publications.

Product Wall

The antibody pairs used in this kit recognize both the alpha and beta chains of mouse HGF

The antibody pair inab100686 recognizes an epitope located somewhere between aa 33-728 (which is whole sequence minus the signal peptide). Therefore the kit is expected to detect the pro (uncleaved) form, the heterodimeric form, the alpha subunit, and ...

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Thank you for contacting us.

Both mouse and human HGF ELISA kits will detect total HGF.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your calls this week and for your patience.

I've heard back from my contact at the lab, and the standard is biologically active. Activity was measured as the ability to stimulate H-thymidine incorporation in 4MBr-5 monkey cells....

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Thank you for contacting Abcam. Unfortunately, epitope mapping has not been performed for this kit so we do not know exactly where the antibodies are binding.  Therefore whether the kit will recognize HGF bound to the c-met receptor or inhibit c-met fr...

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The best kit to use for tissue samples is ab100687 HGF Mouse ELISA Kit - 1 x 96 Well Plate. This is specifically meant for tissue samples. Both of the antibodies in the kit are polyclonal and considering that the homology between mouse and rat is hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"