Overview

  • Product nameHGF Mouse ELISA Kit
    See all HGF kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 100 pg/ml
  • Range
    0.25 ng/ml - 60 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 114.4 100% - 134%
    Tissue Extracts 120.2 110% - 130%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse HGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionHGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
  • Involvement in diseaseDefects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepapoietin A
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A scatter factor
    • Hepatocyte growth factor beta chain
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • Scatter factor
    • SF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

HGF Mouse ELISA Kit images

  • Representative Standard Curve using ab100687

Protocols

References for HGF Mouse ELISA Kit (ab100687)

This product has been referenced in:
  • Demirci C  et al. Loss of HGF/c-Met signaling in pancreatic ß-cells leads to incomplete maternal ß-cell adaptation and gestational diabetes mellitus. Diabetes 61:1143-52 (2012). Read more (PubMed: 22427375) »
  • Araújo TG  et al. Hepatocyte growth factor plays a key role in insulin resistance-associated compensatory mechanisms. Endocrinology 153:5760-9 (2012). Rat . Read more (PubMed: 23024263) »

See all 2 Publications for this product

Product Wall

The best kit to use for tissue samples is ab100687 HGF Mouse ELISA Kit - 1 x 96 Well Plate. This is specifically meant for tissue samples. Both of the antibodies in the kit are polyclonal and considering that the homology between mouse and rat is hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"