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Western blot - HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330)
Western blot - HGFA  Inhibitor 1 antibody - Aminoterminal end (ab28330)
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Product Name 

HGFA Inhibitor 1 antibody - Aminoterminal end

 

See all HGFA Inhibitor 1 antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HGFA Inhibitor 1 - Aminoterminal end

Immunogen 

Synthetic peptide based on the human HGFA Inhibitor 1 sequence.

(Peptide available as ab41268.)

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for HGFA Inhibitor 1 and HAI-1B (a splice variant).

We have a range of domain specific antibodies for this target. For a full list please see all HGFA Inhibitor 1 antibodies

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects a band of approximately 58kDa and 40kDa (predicted molecular weight: 59 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.



Specific protocols
Recommended WB protocol

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> HAIs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Immunology >> Secreted Molecules >> Other secreted molecules

Relevance 

HGFA Inhibitor 1 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 1 also inhibits trypsin, and MTSP-1 (Matriptase), a serine proteinase that also activates HGF. HGFB Inhibitor 1 is a splice variant of HGFA Inhibitor 1, and contains a 16 amino acid insert after the first kringle domain, relative to the HGFA Inhibitor 1 sequence.

 

HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6692    (Human)

Omim

    

605123    (Human)

SwissProt

    

O43278    (Human)

Unigene

    

233950    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HGFA Inhibitor 1 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance!


See below for HGFA Inhibitor 1 antibody - Aminoterminal end images, references, products related to ab28330 and other tools.

 

HGFA Inhibitor 1 antibody - Aminoterminal end images:

Product image  Western blot - HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330)

Western blot - HGFA  Inhibitor 1 antibody - Aminoterminal end (ab28330)

All lanes : HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330) at 1/1000 dilution

Lane 1 : HGFA Inhibitor 1 at 0.05 µg
Lane 2 : HGFA Inhibitor 1 at 0.01 µg
Lane 3 : HGFA Inhibitor 1 at 0.001 µg


Predicted band size : 59 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible cleavage fragment).

 
 

Search PubMed (MEDLINE) for references to HGFA Inhibitor 1

 

HGFA Inhibitor 1 antibody - Aminoterminal end - more information

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HGFA Inhibitor 1 antibody - Aminoterminal end - related products:

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Lysates

 

Immunizing Peptide for HGFA Inhibitor 1 antibody - Aminoterminal end

ab41268

  

HGFA Inhibitor 1 peptide (Aminoterminal end)

 

Compatible Secondaries for HGFA Inhibitor 1 antibody - Aminoterminal end

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other HGFA Inhibitor 1 Antibodies

See all HGFA Inhibitor 1 antibodies (6)...

 

HGFA Inhibitor 1 antibody - Aminoterminal end - other tools:

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