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Western blot - HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330)
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Product Name
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HGFA Inhibitor 1 antibody - Aminoterminal end
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See all HGFA Inhibitor 1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HGFA Inhibitor 1 - Aminoterminal end
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Immunogen
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Synthetic peptide based on the human HGFA Inhibitor 1 sequence.
(Peptide available as ab41268.)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for HGFA Inhibitor 1 and HAI-1B (a splice variant).
We have a range of domain specific antibodies for this target. For a full list please see all HGFA Inhibitor 1 antibodies
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects a band of approximately 58kDa and 40kDa (predicted molecular weight: 59 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> HAIs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Immunology >> Secreted Molecules >> Other secreted molecules
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Relevance
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HGFA Inhibitor 1 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 1 also inhibits trypsin, and MTSP-1 (Matriptase), a serine proteinase that also activates HGF. HGFB Inhibitor 1 is a splice variant of HGFA Inhibitor 1, and contains a 16 amino acid insert after the first kringle domain, relative to the HGFA Inhibitor 1 sequence.
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HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HGFA Inhibitor 1 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HGFA Inhibitor 1 antibody - Aminoterminal end images, references, products related to ab28330 and other tools.
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HGFA Inhibitor 1 antibody - Aminoterminal end images:
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 Western blot - HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330)
All lanes : HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330) at 1/1000 dilution
Lane 1 : HGFA Inhibitor 1 at 0.05 µg
Lane 2 : HGFA Inhibitor 1 at 0.01 µg
Lane 3 : HGFA Inhibitor 1 at 0.001 µg
Predicted band size : 59 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible cleavage fragment).
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Search PubMed (MEDLINE) for references to HGFA Inhibitor 1
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HGFA Inhibitor 1 antibody - Aminoterminal end - more information
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