Anti-HGFA Inhibitor 2 antibody (ab83056)

Overview

  • Product nameAnti-HGFA Inhibitor 2 antibody
    See all HGFA Inhibitor 2 primary antibodies
  • Description
    Rabbit polyclonal to HGFA Inhibitor 2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (MLRCFRQQEN PPLPLGSKVV VLAGLFVMVL ILFLGASMVY LIRVARRNQE) of human HGFA Inhibitor 2 (NP_066925).

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • FunctionInhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.
  • Tissue specificityExpressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.
  • Involvement in diseaseDefects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes.
  • Sequence similaritiesContains 2 BPTI/Kunitz inhibitor domains.
  • DomainThis inhibitor contains two inhibitory domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024025 antibody
    • Bikunin, placental antibody
    • C76321 antibody
    • DIAR3 antibody
    • FLJ45571 antibody
    • HAI-2 antibody
    • HAI2 antibody
    • Hepatocyte growth factor activator inhibitor type 2 antibody
    • Kop antibody
    • Kunitz type protease inhibitor 2 antibody
    • Kunitz-type protease inhibitor 2 antibody
    • Kunitz-type serine protease inhibitor antibody
    • MGC72638 antibody
    • PB antibody
    • Placental bikunin antibody
    • Serine peptidase inhibitor Kunitz type 2 antibody
    • Serine protease inhibitor, Kunitz type, 2 antibody
    • SPINT2 antibody
    • SPIT2_HUMAN antibody
    see all

Anti-HGFA Inhibitor 2 antibody images

  • Anti-HGFA Inhibitor 2 antibody (ab83056) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-HGFA Inhibitor 2 antibody (ab83056)

ab83056 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Listed in the protocol book that I sent you is the western blot protocol we recommend. Since the HGFA Inhibitor 2 protein is located on the cell membrane, we recommend using a NP-40 or RIPA buffer for cell lysis...

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Thank you for contacting Abcam.

Forwarded below is the information that you told me over the phone regarding ab128926 and ab83056. You mentioned ab128926 did produce a positive signal in the THP cell lysate while ab83056 did not produce a posi...

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