• Product nameAnti-HGS antibody
    See all HGS primary antibodies
  • Description
    Rabbit polyclonal to HGS
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 350 and 400 of human HGS (NP_004703.1).

  • Positive control
    • Whole cell lysate from Hela cells.


Associated products


Our Abpromise guarantee covers the use of ab72521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
    • Tissue specificityUbiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
    • Sequence similaritiesContains 1 FYVE-type zinc finger.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
      Contains 1 VHS domain.
    • DomainHas a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
      The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • Post-translational
      Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
      Ubiquitinated by ITCH.
    • Cellular localizationCytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
      • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
      • HGF-regulated tyrosine kinase substrate antibody
      • HGNC:4897 antibody
      • HGS antibody
      • HGS_HUMAN antibody
      • Hrs antibody
      • Human growth factor regulated tyrosine kinase substrate antibody
      • Protein pp110 antibody
      • ZFYVE8 antibody
      see all

    Anti-HGS antibody images

    • Immunoprecipitation/ Western Blot of HGS.
      Lane 1: ab72521 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded).
      Lane 2: Control IgG.
      ab72521 at 1µg/ml for WB.
      Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-HGS antibody (ab72521)

    ab72521 has not yet been referenced specifically in any publications.

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