Overview

  • Product name
    Anti-HGS antibody - C-terminal
    See all HGS primary antibodies
  • Description
    Rabbit polyclonal to HGS - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 611-777 of Human HGS (UniProt ID: O14964).

  • Positive control
    • H1299, HeLa, Mouse brain and Rat brain whole cell lysates; Human colon cancer tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificity
    Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Domain
    Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications
    Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • Cellular localization
    Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Images

  • ab155539 immunoprecipitating HGS in human hTERT-RPE1 whole cell lysate. Cell lysate from 4000000 cells was incubated with primary antibody (1/500 in triton 1% buffer) for 12 hours at 4°C. Western blotting confirmed successful immunoprecipation. Lower 50kDa band corresponds to IgG.

    See Abreview

  • All lanes : Anti-HGS antibody - C-terminal (ab155539) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 86 kDa



    7.5% SDS PAGE
  • Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 86 kDa



    7.5% SDS PAGE
  • Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 86 kDa



    7.5 % SDS-PAGE
  • Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red).
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT-RPE1 cells)
Specification
hTERT-RPE1 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.02% · Temperature: 18°C
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Abcam user community

Verified customer

Submitted May 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (hTERT-RPE1 cells)
Total protein in input
4e+006 cells
Specification
hTERT-RPE1 cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hTERT-RPE1 cells)
Loading amount
5000 cells
Specification
hTERT-RPE1 cells
Gel Running Conditions
Reduced Denaturing (10% acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Apr 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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