Overview

  • Product nameAnti-HGS (phospho Y334) antibody
    See all HGS primary antibodies
  • Description
    Rabbit polyclonal to HGS (phospho Y334)
  • Specificityab62447 detects endogenous levels of HGS only when phosphorylated at tyrosine 334.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human HGS around the phosphorylation site of tyrosine 334 (R-N-YP-W-E).

  • Positive control
    • IHC-P: Human brain tissue ICC/IF: HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab62447 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificityUbiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • DomainHas a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications
    Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • Cellular localizationCytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Anti-HGS (phospho Y334) antibody images

  • Immunohistochemistry analysis of HGS in human brain tissue (paraffin section) using ab62447 (1/50); in the presence or absence of phosphopeptide.
  • Immunofluorescence analysis of HGS in HeLa cells treated with Forskolin (40nM, 15mins); using ab62447 (1/500).

References for Anti-HGS (phospho Y334) antibody (ab62447)

ab62447 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"