Overview

  • Product name
    Anti-Hho1 antibody
  • Description
    Rabbit polyclonal to Hho1
  • Specificity
    This antibody recognizes linker histone from S. Cerevisiae.
  • Tested applications
    Suitable for: ChIP, CHIPseq, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein purified from E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab71833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 22689825
CHIPseq Use at an assay dependent concentration. PubMed: 22689825
WB 1/1000. Predicted molecular weight: 28 kDa.

Target

  • Relevance
    Hho1 could act as an H1-type linker histone. It has been shown to bind DNA.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Hho1p antibody
      • Histone H1, a linker histone required for nucleosome packaging at restricted sites antibody
      • LPI17C antibody
      • Not required for telomeric silencing, basal transcriptional repression, or efficient sporulation antibody
      • Suppresses DNA repair involving homologous recombination antibody
      • YPL127C antibody
      see all

    Images



    • Predicted band size : 28 kDa
    • All lanes : Anti-Hho1 antibody (ab71833) at 1/1000 dilution

      Lane 1 : Wild type S. cerevisiae whole cell lysate
      Lane 2 : Hho1 knock-out S. cerevisiae whole cell lysate
      Lane 3 : S. cerevisiae whole cell lysate expressing Hho1 with tag

      Lysates/proteins at 15 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an anonymous Abreview

      See Abreview

    References

    This product has been referenced in:
    • Brush GS Evidence that histone H1 is dispensable for proper meiotic recombination in budding yeast. BMC Res Notes 8:275 (2015). Read more (PubMed: 26122007) »
    • Sun Z  et al. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J Exp Med 209:1363-77 (2012). WB, ChIP, CHIPseq ; Saccharomyces cerevisiae . Read more (PubMed: 22689825) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (cycling haploid cells)
    Loading amount
    15 µg
    Specification
    cycling haploid cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 23 2013

    Thank you for contacting us.

    I have checked with the lab, butunfortunately, they are unable to provide the antibody in purified, glycerol-free form.

    I am sorry to not have more helpful information, but hope my recent email gave yo...

    Read More

    Thank you for your email.

    The antibody ab71833 comes as whole antiserum and is therefore not purified. The antibody concentration has not been determined as it would not be reflective of the concentration of the specific antibody in the seru...

    Read More

    I now have further information to share with the customer in regards to the protocol used to perform the western blotting experiment presented on the datasheet ofthe Anti-Hho1 antibody (ab71833).


    Theloading buffer used was a standard SD...

    Read More

    Thank you for contacting us.


    The Western blot presented on the datasheet ofAnti-Hho1 antibody (ab71833) wasperformed using extracts prepared by glass bead disruption into 20% TCA (the cell pellet was prepared from a 10 ml mid-log cultur...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up