• Product nameAnti-hHR23A antibody
    See all hHR23A primary antibodies
  • Description
    Rabbit polyclonal to hHR23A
  • SpecificityThis antibody detects endogenous levels of total hHR23A protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from an internal region of human hHR23A

  • Positive control
    • Extracts from LOVO cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab65135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 39 kDa).
ELISA 1/20000.


  • FunctionMultiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
    Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
    Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
  • Sequence similaritiesBelongs to the RAD23 family.
    Contains 2 UBA domains.
    Contains 1 ubiquitin-like domain.
  • DomainThe ubiquitin-like domain mediates interaction with ATXN3.
    The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hHR 23A antibody
    • hHR23A antibody
    • HR23A antibody
    • MGC111083 antibody
    • RAD 23a antibody
    • RAD23 homolog A (S. cerevisiae) antibody
    • RAD23 homolog A antibody
    • RAD23 yeast homolog A antibody
    • RAD23A antibody
    • RD23A_HUMAN antibody
    • UV excision repair protein RAD23 antibody
    • UV excision repair protein RAD23 homolog A antibody
    see all

Anti-hHR23A antibody images

  • All lanes : Anti-hHR23A antibody (ab65135) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells, plus immunising peptide

    Predicted band size : 39 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,36 kDa. We are unsure as to the identity of these extra bands.

References for Anti-hHR23A antibody (ab65135)

ab65135 has not yet been referenced specifically in any publications.

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