Anti-hHR23A antibody [EPR4818] (ab108592)


  • Product nameAnti-hHR23A antibody [EPR4818]
    See all hHR23A primary antibodies
  • Description
    Rabbit monoclonal [EPR4818] to hHR23A
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human hHR23A.

  • Positive control
    • MCF7, HeLa, Raji, and Jurkat cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab108592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionMultiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
    • Sequence similaritiesBelongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.
    • DomainThe ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • hHR 23A antibody
      • hHR23A antibody
      • HR23A antibody
      • MGC111083 antibody
      • RAD 23a antibody
      • RAD23 homolog A (S. cerevisiae) antibody
      • RAD23 homolog A antibody
      • RAD23 yeast homolog A antibody
      • RAD23A antibody
      • RD23A_HUMAN antibody
      • UV excision repair protein RAD23 antibody
      • UV excision repair protein RAD23 homolog A antibody
      see all

    Anti-hHR23A antibody [EPR4818] images

    • All lanes : Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
    • ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG (negative) in green.

    References for Anti-hHR23A antibody [EPR4818] (ab108592)

    ab108592 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (u2os)
    Specification u2os
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Human Cell lysate - whole cell (U2OS)
    Specification U2OS
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 02 2014