Recombinant
RabMAb

Anti-hHR23A antibody [EPR4818] (ab108592)

Overview

  • Product name
    Anti-hHR23A antibody [EPR4818]
    See all hHR23A primary antibodies
  • Description
    Rabbit monoclonal [EPR4818] to hHR23A
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human hHR23A.

  • Positive control
    • MCF7, HeLa, Raji, and Jurkat cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
    • Sequence similarities
      Belongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.
    • Domain
      The ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • hHR 23A antibody
      • hHR23A antibody
      • HR23A antibody
      • MGC111083 antibody
      • RAD 23a antibody
      • RAD23 homolog A (S. cerevisiae) antibody
      • RAD23 homolog A antibody
      • RAD23 yeast homolog A antibody
      • RAD23A antibody
      • RD23A_HUMAN antibody
      • UV excision repair protein RAD23 antibody
      • UV excision repair protein RAD23 homolog A antibody
      see all

    Images

    • All lanes : Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG (negative) in green.

    References

    ab108592 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (u2os)
    Specification
    u2os
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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