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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HIBADH aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P31937
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab175203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.
Flow cytometric analysis of permeabilized HepG2 cells labeling HIBADH with ab175203 at 1/10 dilution (red) compared to a Rabbit IgG (green).
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.
ab175203 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"