Overview

  • Product nameAnti-HIC1 antibody
    See all HIC1 primary antibodies
  • Description
    Rabbit polyclonal to HIC1
  • SpecificityThis antibody is specific for HIC1.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: ALCASERRCS PLCGLDLSKK SPPGSAAPER PLAERELPPR PDSPPSAGPA, corresponding to amino acids 228-277 of Human HIC1

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionTranscriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • Tissue specificityUbiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hic 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

Anti-HIC1 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)

References for Anti-HIC1 antibody (ab49326)

ab49326 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 100000 cells
Specification Fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"