Overview

  • Product nameAnti-HIC1 antibody
    See all HIC1 primary antibodies
  • Description
    Rabbit polyclonal to HIC1
  • SpecificityThis antibody is specific for HIC1.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: ALCASERRCS PLCGLDLSKK SPPGSAAPER PLAERELPPR PDSPPSAGPA, corresponding to amino acids 228-277 of Human HIC1

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionTranscriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • Tissue specificityUbiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hic 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

Anti-HIC1 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)

References for Anti-HIC1 antibody (ab49326)

ab49326 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49326.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"