Overview

  • Product name
  • Description
    Rabbit polyclonal to HIC5
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide (Human). More details of the immunogen are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab42476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 48 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA 1/12500.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
  • Tissue specificity
    Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
  • Sequence similarities
    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Domain
    The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
    The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
  • Post-translational
    modifications
    Phosphorylated by gonadotropin-releasing hormone-activated SRC.
  • Cellular localization
    Cell junction > focal adhesion. Nucleus matrix. Cytoplasm > cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor coactivator 55 kDa protein antibody
    • Androgen receptor coactivator ARA55 antibody
    • Androgen receptor-associated protein of 55 kDa antibody
    • ARA55 antibody
    • Hic-5 antibody
    • Hydrogen peroxide-inducible clone 5 protein antibody
    • Tgfb1i1 antibody
    • TGFI1_HUMAN antibody
    • Transforming growth factor beta 1 induced transcript 1 antibody
    • Transforming growth factor beta-1-induced transcript 1 protein antibody
    • TSC 5 antibody
    see all

Anti-HIC5 antibody images

  • Anti-HIC5 antibody (ab42476) at 1 µg/ml + Jurkat cell lysate

    Predicted band size : 48 kDa
  • Ab42476 at 4.0µg/ml staining Alveolar cells of human lung. Paraffin embedded tissue. Magnification 400X
  • ICC/IF image of ab42476 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42476, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-HIC5 antibody (ab42476)

This product has been referenced in:
  • Hornigold N  et al. Inhibition of collagen I accumulation reduces glomerulosclerosis by a Hic-5-dependent mechanism in experimental diabetic nephropathy. Lab Invest 93:553-65 (2013). Rat . Read more (PubMed: 23508044) »
  • Hornigold N  et al. Upregulation of Hic-5 in glomerulosclerosis and its regulation of mesangial cell apoptosis. Kidney Int 77:329-38 (2010). IHC-P ; Rat . Read more (PubMed: 20010548) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Tissue sections (uterus at 7.5 day of pregnancy)
Specification
uterus at 7.5 day of pregnancy
Permeabilization
No
Fixative
Methanol
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Submitted Nov 07 2014

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabeads
Sample
Human Cell lysate - whole cell (endothelial)
Specification
endothelial
Total protein in input
3e+006 cells
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Verified customer

Submitted Feb 13 2014

Application
ChIP
Sample
Mouse Cell lysate - nuclear (myeloid progenitors)
Specification
myeloid progenitors
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 12 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
my gene of interest
Negative control
Chromosome 2 inactive DNA
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Verified customer

Submitted Apr 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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