• Product name
  • Description
    Rabbit polyclonal to HIC5
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PRSGAPKERP AEPLTPPPSY GHQPQTGSGE SSGASGDKDH LYSTVCKPRS) of human HIC5 (NP_057011).

  • Positive control
    • Transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab83273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
  • Tissue specificity
    Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
  • Sequence similarities
    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Domain
    The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
    The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
  • Post-translational
    Phosphorylated by gonadotropin-releasing hormone-activated SRC.
  • Cellular localization
    Cell junction > focal adhesion. Nucleus matrix. Cytoplasm > cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor coactivator 55 kDa protein antibody
    • Androgen receptor coactivator ARA55 antibody
    • Androgen receptor-associated protein of 55 kDa antibody
    • ARA55 antibody
    • Hic-5 antibody
    • Hydrogen peroxide-inducible clone 5 protein antibody
    • Tgfb1i1 antibody
    • TGFI1_HUMAN antibody
    • Transforming growth factor beta 1 induced transcript 1 antibody
    • Transforming growth factor beta-1-induced transcript 1 protein antibody
    • TSC 5 antibody
    see all

Anti-HIC5 antibody images

  • Anti-HIC5 antibody (ab83273) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling HIC5 with ab83273 at 5.0µg/ml.

References for Anti-HIC5 antibody (ab83273)

ab83273 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83273.
Please use the links above to contact us or submit feedback about this product.


Sign up