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Recombinant fragment corresponding to Human HIF-1-alpha aa 432-528.
Use hypoxia induced cells as postives control. Hypoxia can be induced with treatment using certain agents e.g. CoCl2 or DFO, etc so proper sample preparation is critical.
Our Abpromise guarantee covers the use of ab80544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"