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Recombinant fragment corresponding to Human HIF-1 alpha aa 530-826.
Use hypoxia induced cells as postives control. Hypoxia can be induced with treatment using certain agents e.g. CoCl2 or DFO, etc so proper sample preparation is critical.
Our Abpromise guarantee covers the use of ab80521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|EMSA||Use a concentration of 1 mg/ml.|
ab80521 has not yet been referenced specifically in any publications.