Anti-HIF-1-alpha [H1alpha67] antibody - ChIP Grade (ab463)
- Product nameAnti-HIF-1-alpha [H1alpha67] antibody - ChIP GradeSee all HIF-1-alpha primary antibodies ...
- DescriptionMouse monoclonal [H1alpha67] to HIF-1-alpha - ChIP Grade
- Tested applicationsICC/IF, IP, WB, Gel supershift assays, IHC-P, ELISA, ChIP, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Cow, Dog, Human, Pig, Bird, Ferret, Monkey
Fusion protein corresponding to Human HIF-1-alpha aa 432-528.
Database link: Q16665
- Positive control
- Glioblastoma multiforme.
- Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituents: Tris glycine, 0.87% Sodium chloride
- Concentration information loading...
- PurityProtein A purified
- Purification notesProtein A purified from cell culture supernatant.
- Clone numberH1alpha67
Our Abpromise guarantee covers the use of ab463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: 1/100 - 1/500.|
|WB||WB: 1/500 - 1/1000.|
|Gel supershift assays||GSA: Use at an assay dependent concentration.|
|IHC-P||IHC-P: 1/100 - 1/300. Antigen retrieval is not essential but may optimise staining.|
|ELISA||ELISA: Use at an assay dependent concentration.|
|ChIP||ChIP: Use at an assay dependent concentration.|
|Flow Cyt||Flow Cyt: Use at an assay dependent concentration.|
- FunctionFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
- Tissue specificityExpressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
- DomainContains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
modificationsIn normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
- Cellular localizationCytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
- Entrez Gene: 281814 Cow
- Entrez Gene: 480348 Dog
- Entrez Gene: 3091 Human
- Entrez Gene: 15251 Mouse
- Entrez Gene: 396696 Pig
- Entrez Gene: 29560 Rat
- Entrez Gene: 443519 Sheep
- Omim: 603348 Human
- SwissProt: Q9XTA5 Cow
- SwissProt: Q16665 Human
- SwissProt: Q61221 Mouse
- SwissProt: O35800 Rat
- Unigene: 597216 Human
- Unigene: 3879 Mouse
- Unigene: 446610 Mouse
- Unigene: 10852 Rat
- ARNT interacting protein antibody
- ARNT-interacting protein antibody
- Basic helix loop helix PAS protein MOP1 antibody
- Basic-helix-loop-helix-PAS protein MOP1 antibody
- bHLHe78 antibody
- Class E basic helix-loop-helix protein 78 antibody
- HIF 1A antibody
- HIF 1alpha antibody
- HIF-1-alpha antibody
- HIF1 A antibody
- HIF1 Alpha antibody
- HIF1 antibody
- HIF1-alpha antibody
- HIF1A antibody
- HIF1A_HUMAN antibody
- Hypoxia inducible factor 1 alpha antibody
- Hypoxia inducible factor 1 alpha isoform I.3 antibody
- Hypoxia inducible factor 1 alpha subunit antibody
- Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
- Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody
- Hypoxia inducible factor1alpha antibody
- Hypoxia-inducible factor 1-alpha antibody
- Member of PAS protein 1 antibody
- Member of PAS superfamily 1 antibody
- Member of the PAS Superfamily 1 antibody
- MOP 1 antibody
- MOP1 antibody
- PAS domain-containing protein 8 antibody
- PASD 8 antibody
- PASD8 antibody
Anti-HIF-1-alpha [H1alpha67] antibody - ChIP Grade images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling HIF-1-alpha with ab463.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Dog breast cancer tissue sections labeling HIF-1-alpha with ab463 at 1/500. Tissue was fixed with formaldehyde and blocked with a peroxidase block for 5 minutes at 25°C; antigen retrieval was by heat mediation with EDTA buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 30 minutes at 25°C. A biotin-conjugated Mouse anti-mouse IgG polyclonal was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling HIF-1-alpha with ab463.
All lanes : Anti-HIF-1-alpha [H1alpha67] antibody - ChIP Grade (ab463)
Lane 1 : Cobalt chloride treated COS-7 nuclear extracts
Lane 2 : Unteated COS-7 nuclear extracts
References for Anti-HIF-1-alpha [H1alpha67] antibody - ChIP Grade (ab463)
This product has been referenced in:
- Yu TM et al. Expression of hypoxia-inducible factor-1a (HIF-1a) in infiltrating inflammatory cells is associated with chronic allograft dysfunction and predicts long-term graft survival. Nephrol Dial Transplant 28:659-70 (2013). IHC-P ; Human . Read more (PubMed: 23028107) »
- Cannino G et al. Glucose modulates respiratory complex I activity in response to acute mitochondrial dysfunction. J Biol Chem 287:38729-40 (2012). WB ; Human . Read more (PubMed: 23007390) »