Anti-HIF Prolyl Hydroxylases antibody (ab94626)

Overview

  • Product name
    Anti-HIF Prolyl Hydroxylases antibody
    See all HIF Prolyl Hydroxylases primary antibodies
  • Description
    Rabbit polyclonal to HIF Prolyl Hydroxylases
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (LGNGWWMTPE SIQEMYAAIK ADPDGDGVLS LQEFSNMDLR DFHKYMRSHK) of Human HIF Prolyl Hydroxylases (NP_808808).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificity
    Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
  • Sequence similarities
    Contains 2 EF-hand domains.
    Contains 1 Fe2OG dioxygenase domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGLN4 antibody
    • FLJ20262 antibody
    • HIF prolyl hydroxylase PH4 antibody
    • HIF-PH4 antibody
    • HIF-prolyl hydroxylase 4 antibody
    • HIFPH4 antibody
    • HPH-4 antibody
    • Hypoxia inducible factor prolyl 4 hydroxylase antibody
    • Hypoxia inducible factor prolyl hydroxylase antibody
    • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
    • P4H TM antibody
    • P4H with transmembrane domain antibody
    • P4H-TM antibody
    • P4htm antibody
    • P4HTM_HUMAN antibody
    • PH 4 antibody
    • PH4 antibody
    • PHD4 antibody
    • Proline 4 hydroxylase antibody
    • Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibody
    • Prolyl hydroxlase domain containing 4 antibody
    • Transmembrane prolyl 4-hydroxylase antibody
    see all

Images

  • Anti-HIF Prolyl Hydroxylases antibody (ab94626) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa

References

ab94626 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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