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Product name
Anti-HIF1 beta antibody
See all HIF1 beta products (11) ...
Description
Rabbit polyclonal to HIF1 beta - ChIP Grade
Conjugation notes
-
Specificity
This antibody is specific for HIF-1 beta/ARNT. It is not known if ab2 crossreacts with ARNT2 (which is related but a different gene product).
Tested applications
ChIP/Chip, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Cow, Human, Ferret
Immunogen
Fusion protein, containing amino acids 496-789 of Human HIF-1 beta.
Positive control
MCF7 human nuclear extracts
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Nucleotide metabolism
Cancer >> Cancer Metabolism >> Response to hypoxia
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
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ChIP - HIF1 beta antibody (ab2)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP/Chip: Use at an assay dependent dilution. PubMed: 20395365
IP: Use at an assay dependent dilution.
WB: 1/2000. Predicted molecular weight: 92 kDa.Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations.
ChIP: Use 0.2 µg for µg of chromatin.
Target
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP27540
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- nuclear translocator antibody
- ARNT antibody
- ARNT protein antibody
see all
Database links
- Entrez Gene: 281010 Cow
- Entrez Gene: 405 Human
- Entrez Gene: 11863 Mouse
- Entrez Gene: 25242 Rat
- Omim: 126110 Human
- SwissProt: Q9BE97 Cow
- SwissProt: P27540 Human
- SwissProt: P53762 Mouse
see all
Anti-HIF1 beta antibody images:
ChIP - HIF1 beta antibody (ab2)
X-ChIP with ab2 showing enrichment of HIF1 beta at a region upstream of transcription start site of the VEGFA gene. Chromatin was extracted from a glioma cell line and the proteins were cross-linked to DNA with formaldehyde for 15 minutes. The immunoprecipitation step involved incubation of the cross-linked chromatin with ab2 for 16 hours at 4°C. Please see abreview by Genpathway for additional details.
This image is courtesy of an Abreview submitted by Genpathway Inc
References for Anti-HIF1 beta antibody (ab2)
This product has been referenced in:
- Xu J et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes Dev 24:783-98 (2010). WB, ChIP/Chip; Human.Read more (PubMed: 20395365) »
- Echevarría M et al. Development of cytosolic hypoxia and hypoxia-inducible factor stabilization are facilitated by aquaporin-1 expression. J Biol Chem 282:30207-15 (2007). WB; Rat.Read more (PubMed: 17673462) »
See all 2 publications for this product
Publishing research using ab2? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"